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Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength

Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength

Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength
Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength

Large Image :  Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength

Product Details:
Place of Origin: CHINA
Brand Name: shengtai Instrument
Model Number: ST-2000A
Payment & Shipping Terms:
Minimum Order Quantity: 1
Payment Terms: T/T

Visual Cloth Board Feed Testing Instrument Mycotoxin Analyzer 400-900nm Wavelength

Description
Light Source:: DC12V 22W Imported Halogen Lamp Wavelength Range:: 400-900nm
Reading Range:: 0-4.000Abs Resolution:: 0.001Abs
Indication Error:: ≤±0.01Abs Stability:: ≤±0.003Abs
High Light:

automatic kjeldahl nitrogen analyzer

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hardness testing machine

St-2000a mycotoxin analyzer is a necessary analytical instrument for the analysis of aflatoxin and enzyme-linked immunity.
Using solid phase enzyme-linked immunosorbent ELISA principle, namely, enzyme-linked immunoassay, composed of the instrument and the B1 kit 2 big, can set limit to, quantitative determination of the content of aflatoxin B1 in samples (such as with other kits, measurable other toxins), widely used in food, food, feed, oil, dairy products, medicine, beverage, wine and other products of aflatoxin B1 and other detection of mycotoxin.
Performance features:
1, display operation: color LCD screen, visual cloth board, display the whole board information, touch screen operation
2. Vibration plate function: the speed and time of the 3-stage vibration plate can be adjusted from 0 to 255 seconds
3. Working station: a professional software of enzyme label analyzer, which can store 500 sets of programs and 100,000 sample results, provides a variety of calculation modes such as absorbance, linear equation, logarithmic equation, quadratic equation, cubic equation, absorbance percent-concentration logarithmic equation, spline function, inhibition rate, etc
Technical parameters:
Light source: DC12V 22W imported halogen lamp
Wavelength range: 400-900nm
Filter: standard with 405, 450, 492, 630nm, other wavelengths optional
Reading range: 0-4.000Abs
Resolution: 0.001Abs
Indication error: ≤±0.01Abs
Stability: ≤±0.003Abs
Repeatability: ≤0.3%
Size: 400 mm (L) (W) * 200 mm * 260 mm (H)


Principle and application
This kit USES an indirect competitive ELISA method to detect Aflatoxin B1 (AFB1) in grain and feed samples. The kit consists of an enzyme label plate, horseradish enzyme marker, antibody, standard product and other supporting reagents precoated with coupled antigen.
Test, to join the standard and sample solution, aflatoxin B1 and enzyme label plate sample pre package is coupling antigen antibody of aflatoxin B1, competition after joining enzyme markers, with TMB substrate color, sample absorbance value and content of aflatoxin B1 contained negative correlation, compared with the standard curve can be concluded that the residues of aflatoxin B1 in the sample.
2. Technical indicators
2.1 kit sensitivity: 0.03 PPB (ng/ml)
2.2 reaction mode: 25℃, 30min ~ 15min
2.3 detection limit:
Grain.....................................................................
0.15 PPB
Compound feed........................
0.3 PPB
Cooking oil, peanuts...
0.6 PPB
Sauce, wheat and other feeds............
0.3 PPB
Beer.....................................................................
0.3 PPB
Wine, soy sauce, vinegar...
0.15 PPB
2.4 cross reaction rate:
Aflatoxin B1..................
100%
2.5 sample recovery:
Grain and compound feed............
85% plus or minus 15%
Peanut..................................................................
82 + / - 15%
Cooking oil...............................................................
85 + / - 15%
Sauce, wheat and other feeds......
83 + / - 15%
Beer...............................................................
84 + / - 15%
Wine, soy sauce, vinegar...
87 + / - 15%
3 kit composition
Enzyme label plate...................................................
96 hole
Standard liquid (black cover) : 1ml each
0ppb, 0.03 PPB, 0.06 PPB, 0.12 PPB, 0.24 PPB, 0.48 PPB
High standard liquid: 100ppb............
1 ml
Enzyme marker (red cap)......
5.5 ml
Antibody working fluid (blue cap)......
5.5 ml
Substrate A (white cap)..................
6 ml
Substrate B (black cap)......
6 ml
Termination fluid (yellow cap)........................
6 ml
20X concentrated washing liquid (white cover)......
40 ml
Manual...............................................................
1
Equipment and reagents required
4.1 instruments: aflatoxin tester, printer, homogenizer, nitrogen blow-drying device, oscillator, centrifuge, scale pipetting tube, balance (sensitivity: 0.01g)
4.2 micropipette: single channel 20 l-200 l, 100 l-1000 l, multiple channels 300 l
4.3 reagent: methanol, n-hexane, trichloromethane or dichloromethane
5. Sample pretreatment
5.1 instructions before sample processing:
The experimental apparatus must be clean and disposable suction head must be used to avoid contamination from interfering with the experimental results.
5.2 with liquid:
Liquid 1: sample extract
70% methanol, V methanol :V deionized water = 7:3.
5.3 sample pretreatment steps:
5.3.1 grain treatment method
1) weigh 2g crushed samples into 50ml centrifuge tube, add 5ml sample extract, oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) take 0.5ml supernatant, add 0.5ml deionized water and mix well;
3) set 50 living-room liters for analysis.
Sample dilution factor: 5 lower limit of detection: 0.15 PPB
5.3.2 sample treatment methods of corn husk and wheat bran with strong water absorption
1) weigh 2g crushed samples into 50ml centrifuge tube, add 20ml sample extract, oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) take 0.5ml supernatant, add 0.5ml deionized water and mix well;
(samples with extremely high levels of toxins can be diluted with 35% methanol before testing.
For example, 0.1ml of the mixture in 2) was mixed with 35% methanol 0.9ml. The dilution ratio of the final sample was 200, and the lower limit of detection was 6ppb.
3) set 50 living-room liters for analysis.
Sample dilution factor: 20 lower limit of detection: 0.6 PPB
Note: since the distribution of toxin in the sample is non-uniform, multiple samples should be sampled and thoroughly mixed, and then 2g should be taken for the test.
5.3.3 treatment method of compound feed
1) weigh 2g crushed samples into 50ml centrifuge tube, add 10ml sample extract, and oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) take 0.5ml supernatant, add 0.5ml deionized water and mix well;
3) set 50 living-room liters for analysis.
Sample dilution factor: 10 lower limit of detection: 0.3 PPB
5.3.4 treatment of edible oil, peanut and high fat feed
1) weigh 2g crushed samples into 50ml centrifuge tube, add 8ml n-hexane and 10ml sample extract, and oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) remove the upper liquid, take 0.5ml of the lower liquid and add 0.5ml of deionized water, mix it, and then take 0.5ml of the mixed liquid and add 0.5ml of 35% methanol, oscillate for 30S;
3) set 50 living-room liters for analysis.
Sample dilution factor: 20 lower limit of detection: 0.6 PPB
5.3.5 sauce, wheat, cookies, cakes and other food or seasoning, as well as feed concentrate and other feed treatment methods
1) weigh 2g crushed samples into 50ml centrifuge tube, add 10ml sample extract, and oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) take 2ml supernatant, add 4ml trichloromethane (or dichloromethane), and oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
3) transfer the upper liquid to another container, reserve the lower liquid for use, add 4ml trichloromethane (or dichloromethane) to the upper liquid, oscillate and mix fully for 5 minutes, at room temperature of 4000 RPM/separation center for 10 minutes;
4) remove the upper liquid, combine the lower liquid twice and mix well;
5) take 2ml of the combined lower liquid and dry it under nitrogen at 50-60℃;
6) add 0.5ml of sample extract to fully dissolve the drying substance, and then add 0.5ml of deionized water to mix well;
7) set 50 yl to analyze.
Sample dilution factor: 10 lower limit of detection: 0.3 PPB
5.3.6 beer treatment method
1) stir the beer thoroughly (remove CO2), take 2ml beer sample, add 1ml distilled water, then add 7ml methanol, and oscillate for 5 minutes;
2) take 0.5ml of the sample solution after mixing and add 0.5ml of deionized water to mix well;
3) set 50 living-room liters for analysis.
Sample dilution factor: 10 lower limit of detection: 0.3 PPB
5.3.7 treatment of wine, soy sauce and vinegar
1) take a 2ml sample, add 2ml distilled water, then add 10ml trichloromethane (or dichloromethane), and oscillate for 5 minutes at room temperature of 4000 RPM/separation center for 10 minutes;
2) take 1ml of the lower liquid and dry it under nitrogen at 50-60℃;
3) add 0.5ml of sample extract to fully dissolve the drying substance, then add 0.5ml of deionized water and mix well;
5) set 50 living-room liters for analysis.
Sample dilution factor: 5 lower limit of detection: 0.15 PPB
6. Steps of elisa test
The required reagent was taken out from the refrigerated environment at 4℃ and balanced at room temperature for more than 30min. There might be crystals in the refrigerated washing solution and they should be fully dissolved at room temperature. All the liquid reagents should be shaken well before use.
Take out the required number of microporous plates and frames, put the unused microporous plates into a self-sealing bag and store them at 2-8℃.
Before the experiment, the 20× concentrated washing solution was diluted by 20 times with deionized water.
6.1 serial number: the micropores corresponding to the samples and the standard are numbered in sequence, and each sample is made with 2 holes parallel to the standard, and the positions of the standard holes and the sample holes are recorded.
6.2 sample addition reaction: standard product or sample 50 l/ well was added into the respective micropores, followed by enzyme marker 50 l/ well, followed by antibody working liquid 50 l/ well, the plate was sealed with a cover plate membrane, gently shaken for 5 seconds, mixed, and reacted at 25℃ for 30 minutes.
6.3 washing and scrubbing: carefully uncover the cover film, shake and dry the liquid in the hole, fully wash the hole 5 times with work washing liquid 250 l/ hole at an interval of 30 seconds, and pat dry with absorbent paper (the bubbles that have not been cleaned after patting dry can be punctured with clean nozzle).
6.4 color rendering: add substrate solution A 50 l to each well, then add substrate solution B 50 l, gently oscillate for 5 seconds and mix, and color rendering at 25℃ for 15 minutes in dark.
6.5 termination: add termination liquid 50 l into each well, mix it with slight oscillation, and terminate the reaction.
6.6 absorbance value: the absorbance value of each well was determined at 450nm with aflatoxin analyzer (dual wavelength 450/630nm is recommended).
The assay should be completed within 10 minutes of reaction termination
6.7 the st-2000a automatic aflatoxin tester automatically completed the measurement and produced the results.

Contact Details
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